分離済み変異体

tm1297

Allele Nametm1297
BalanceNot Required
OutCrossNot Accepted
Sequence NameF29C4.6
Gene Nametut-1
Worm BaseAllele Name tm1297
Gene Name tut-1
Sequence F29C4.6
Phenotype Information from the receiver is posted in the form of a "researcher : phenotype" homozygous viable. Dr. R. Plasterk: reduced brood size, sterile at 25C. Dr. M. Peter: Nature 458, 228 (2009). Dr. A. Bystrom: PLoS Genetics 5, e1000561 (2009)
Mutation site Please see gene structure to locate the deletion in relation to exon(s) 10824/10825-G-11650/11651 (826 bp deletion + 1 bp insertion)
ChromosomeIV
Putative gene structurecomplement(join(9653..9902, 10621..11327, 11781..11945))
Map position-26.96
Balancer
Map position of balancer
Sequence of primersExtFwd:CAACGTCGGCATTTCGACCT,ExtRev:GACAAGCTGGCATCATGTAG,IntRev:TAACCGTACCCGGCGGCATT,IntFwd:CGATAGGCATTCGGTGCGTA
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
Meyer DH, Schumacher B.
BiT age: A transcriptome-based aging clock near the theoretical limit of accuracy.
Aging Cell 2021 20(3) e13320 
[ PubMed ID = 33656257 ] [ RRC reference ]

Fernandes De Abreu DA, Salinas-Giegé T, Drouard L, Remy JJ.
Alanine tRNAs Translate Environment Into Behavior in Caenorhabditis elegans.
Front Cell Dev Biol 2020 8 571359 
[ PubMed ID = 33195203 ] [ RRC reference ]

Kawamura K, Maruyama IN.
Forward Genetic Screen for Caenorhabditis elegans Mutants with a Shortened Locomotor Healthspan.
G3 (Bethesda) 2019 9(8) 2415-2423 
[ PubMed ID = 31213517 ] [ RRC reference ]

Nedialkova DD, Leidel SA.
Optimization of Codon Translation Rates via tRNA Modifications Maintains Proteome Integrity.
Cell 2015 161(7) 1606-18 
[ PubMed ID = 26052047 ] [ RRC reference ]

Chen C, Tuck S, Byström AS.
Defects in tRNA modification associated with neurological and developmental dysfunctions in Caenorhabditis elegans elongator mutants.
PLoS Genet 2009 5(7) e1000561 
[ PubMed ID = 19593383 ] [ RRC reference ]

Leidel S, Pedrioli PG, Bucher T, Brost R, Costanzo M, Schmidt A, Aebersold R, Boone C, Hofmann K, Peter M.
Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA.
Nature 2009 458(7235) 228-32 
[ PubMed ID = 19145231 ] [ RRC reference ]

Dewez M, Bauer F, Dieu M, Raes M, Vandenhaute J, Hermand D.
The conserved Wobble uridine tRNA thiolase Ctu1-Ctu2 is required to maintain genome integrity.
Proc Natl Acad Sci U S A 2008 105(14) 5459-64 
[ PubMed ID = 18391219 ] [ RRC reference ]