分離済み変異体

tm1094

Allele Nametm1094
BalanceNot Required
OutCrossNot Accepted
Sequence NameC01G5.2
Gene Nameprg-2
Worm BaseAllele Name tm1094
Gene Name prg-2
Sequence C01G5.2
Phenotype Information from the receiver is posted in the form of a "researcher : phenotype" homozygous viable. Dr. C. Mello, Cell 127, 747-457 (2006). Dr. E. Miska; Mol Cell 31, 79-90 (2008).
Mutation site Please see gene structure to locate the deletion in relation to exon(s) 14468/14469-AAACAAGTGTTTAACAATTAAACAAGT-15533/15534 (1065 bp deletion + 27 bp insertion)
ChromosomeIV
Putative gene structurecomplement(join(13134..13289, 13335..13521, 13564..13801, 13849..14023, 14071..14435, 14479..14920, 14968..15210, 15380..15568, 15613..15786))
Map position3.2
Balancer
Map position of balancer
Sequence of primersIntRev:CGGGCCAACCATCAAAATGA,ExtRev:CTGGGAACTATCCAACCCGA,IntFwd:ATCTTACTTCGGACGAAGTG,ExtFwd:CAGCACCATCTCTGTAGAGA
Distributed lab
DepositorDr. S. Mitani
References Please submit your publication
Ow MC, Nishiguchi MA, Dar AR, Butcher RA, Hall SE.
RNAi-dependent expression of sperm genes in ADL chemosensory neurons is required for olfactory responses in Caenorhabditis elegans.
Front Mol Biosci 2024 11 1396587 
[ PubMed ID = 39055986 ] [ RRC reference ]

Shukla A, Perales R, Kennedy S.
piRNAs coordinate poly(UG) tailing to prevent aberrant and perpetual gene silencing.
Curr Biol 2021 31(20) 4473-4485.e3 
[ PubMed ID = 34428467 ] [ RRC reference ]

Pereira AG, Gracida X, Kagias K, Zhang Y.
C. elegans aversive olfactory learning generates diverse intergenerational effects.
J Neurogenet 2020 34(3-4) 378-388 
[ PubMed ID = 32940103 ] [ RRC reference ]

Zhou X, Xu F, Mao H, Ji J, Yin M, Feng X, Guang S.
Nuclear RNAi contributes to the silencing of off-target genes and repetitive sequences in Caenorhabditis elegans.
Genetics 2014 197(1) 121-32 
[ PubMed ID = 24532782 ] [ RRC reference ]

Batista PJ, Ruby JG, Claycomb JM, Chiang R, Fahlgren N, Kasschau KD, Chaves DA, Gu W, Vasale JJ, Duan S, Conte D Jr, Luo S, Schroth GP, Carrington JC, Bartel DP, Mello CC.
PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans.
Mol Cell 2008 31(1) 67-78 
[ PubMed ID = 18571452 ] [ RRC reference ]

Wang G, Reinke V.
A C. elegans Piwi, PRG-1, regulates 21U-RNAs during spermatogenesis.
Curr Biol 2008 18(12) 861-7 
[ PubMed ID = 18501605 ] [ RRC reference ]